Embryo 18-20h RNA-Seq Multi mapper

Dm Dev Timecourse Expression RNA-seq em18-20hr multiply mapping reads (Celniker project, Celniker subgroup)

Details

Unstranded libraries were created from polyA+ RNA isolated from staged em18-20hr y cn bw sp D. melanogaster flies, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned with TopHat against the r5 genome. Tracks show read alignments and density.
Total read count: 126586497
Mapped read count: 95381501
Alignment rate: 75%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Reads from Illumina GAIIx were aligned to the genome sequence using TopHat.

Protocols

1. Data Analysis: TopHat RNAseq alignment

1. External Links: SRR023503, SRR023599, SRR023673, SRR023682, SRR023686, SRR023699, SRR023700, SRR024215, SRR027114, SRR035222, SRR035403

Related modENCODE submissions:

• modENCODE_4444: Dm Dev Timecourse Expression RNA-seq em18-20hr sequences

Release Date: 2012-02-07