L3 PS3-6 RNA-Seq Unique mapper

Dm Developmental Timecourse Expression RNA-seq L3_PS3-6 uniquely mapping reads (Celniker project, Graveley subgroup)

Details

Unstranded libraries were created from polyA+ RNA isolated from staged L3 PS3-6 y cn bw sp D. melanogaster flies, sequenced using Illumina GAIIx with single and/or paired-end modules, and aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 132689476
Mapped read count: 55464347
Alignment rate: 41%

General Description

RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Unstranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

  1. Data Analysis: Stranded paired-end alignment
  1. External Links: GEO:TMPID:SRR023505, GEO:TMPID:SRR023676, GEO:TMPID:SRR023683, GEO:TMPID:SRR023690, GEO:TMPID:SRR023692, GEO:TMPID:SRR023742, GEO:TMPID:SRR027108, SRR023505, SRR023676, SRR023683, SRR023690, SRR023692, SRR023742, SRR027108

Related modENCODE submissions:


Release Date: 2012-03-20