Kc WT Repliseq (MacAlpine project, MacAlpine subgroup)
General DescriptionThe relative time of replication for all unique sequences in the Drosophila genome was determined by
sequencing replication intermediates that were differentially labeled with BrdU during S-phase progression. Briefly, cells were pulse labeled with BrdU and sorted into early, middle and late S-phase fractions. BrdU labeled sequences from each fraction of S-phase were enriched by immunoprecipitation and sequenced on the Illumina platform.
Release Date: 2011-05-20