Male Eclosion + 1 days RNA-Seq Multi mapper

Dm adult male, eclosion + 1 day RNA-Seq, multi mappers (Celniker project)

Details

RNA-seq was performed on a single biological sample of D. melanogaster (y cn bw sp) adult males 1 day past eclosion, using a combination of single- and paired-end sequencing. Sequences were aligned to the FlyBase r5 genome using TopHat. Track displays alignment read density.
Uniquely mapped reads: 81926609
Multiply mapped reads: 2245937
Total reads sequenced: 111635373

General Description

RNA-seq analysis was performed on poly(A)+ RNA from 30 developmental stages spanning the life cycle of D. melanogaster, from 0-2hr embryos through 30-day male and female adults. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A)+ RNA and library construction were performed in the Brenton Graveley lab. Libraries were distributed among 5 labs in the Drosophila Transcriptome group for sequencing. The Susan Celniker lab performed paired-end sequencing exclusively (2x76 nt) . The Brenton Graveley, Tom Gingeras and Michael Brent labs performed single-end sequencing exclusively (1x76 nt). The Brian Oliver lab performed both single- and paired-end sequencing. All labs used the same Illumina GAII platform and called bases using the Illumina processing pipeline. Fastq files were generated using pipeline version 1.3 before 5/07/09 and using version 1.4 after that date. Reads were aligned to the genome sequence using Tophat v.1.0.10.

Protocols

1. Growth and isolation: mRNA purification from total RNA
2. Sample preparation: Sample prep
3. Other Protocols: Paired-end Illumina Sequencing Single-end Illumina Sequencing TopHat alignment

Sample Details

1. Samples: Celniker/RNA:370
2. External Links: SRR023543 SRR023648 SRR023664 SRR023678 , and 24 more.

Related modENCODE submissions:

• DCC-574: Dev Timecourse TotalRNA isolation
• DCC-98: Dm_y[1]cn[1] bw[1] sp[1]_adult_Male_1dayPostEclosion_TotalRNA_p200_368-370-372_38bp

Release Date: 2010-06-02