Males+Females, Eclosion+1 day, digestive system Unique mapper

Dm Tissue Expression RNA-seq mixed males and females eclosion + 1 day digestive system uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)

Details

Reads sequenced from stranded RNA libraries made from Oregon-R Drosophila melanogaster mixed males and females eclosion + 1 day digestive system were aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 59837886
Mapped read count: 50593030
Alignment rate: 84%

General Description

Stranded RNA-seq analysis was performed on poly(A)+ RNA from various tissues dissected from various stages of D. melanogaster. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

1. Data Analysis: Stranded paired-end alignment

1. External Links: SRR070398, SRR070394

Related modENCODE submissions:

• modENCODE_4252 Dm Tissue Expression RNA-seq mixed males and females eclosion + 1 day digestive system sequences

Release Date: 2012-02-24