L3, wandering stage, imaginal discs Unique mapper

Dm Tissue Expression RNA-seq third instar larvae wandering stage imaginal discs uniquely mapping reads Dmel r5 alignment (Celniker project, Graveley subgroup)

Details

Reads sequenced from stranded RNA libraries made from Oregon-R Drosophila melanogaster third instar larvae wandering stage imaginal discs were aligned to the D. melanogaster r5 genome using Bowtie and predicted splice junctions with SPA (allowing only unique alignments). Tracks show read alignments and density.
Total read count: 415010002
Mapped read count: 253191420
Alignment rate: 61%

General Description

Stranded RNA-seq analysis was performed on poly(A)+ RNA from various tissues dissected from various stages of D. melanogaster. Stranded reads from Illumina GAIIx and HiSeq sequencing were aligned to the D. melanogaster r5 genome using Bowtie (Langmead, 2010) and SPA (Graveley et al., 2011)

Protocols

1. Data Analysis: Stranded paired-end alignment

1. External Links: SRR070392, SRR070393, SRR111884, SRR111885, SRR350962, SRR350963

Related modENCODE submissions:

• modENCODE_4261: Dm Tissue Expression RNA-seq third instar larvae wandering stage imaginal discs sequences

Release Date: 2012-02-24