L2 N2 hermaphrodite
mid-L2 N2 hermaphrodite small ncRNA RNA-Seq (Waterston project, Slack subgroup)
small RNAs (less than 200nt) were isolated from stress tested mid-L2 animals, and sequenced using Illumina technology. Sequences were aligned to the WS190 genome using SOAP, converted to SAM, then lifted to WS220. Tracks show alignments/read density.
We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during development in C. elegans. RNAs were prepared from different time points during development of C. elegans hermaphrodites and males, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.
Release Date: 2009-11-18