L2 N2 hermaphrodite

mid-L2 N2 hermaphrodite small ncRNA RNA-Seq (Waterston project, Slack subgroup)

Details

small RNAs (less than 200nt) were isolated from stress tested mid-L2 animals, and sequenced using Illumina technology. Sequences were aligned to the WS190 genome using SOAP, converted to SAM, then lifted to WS220. Tracks show alignments/read density.
Total read count: 10031813
Mapped read count: 6049419
Alignment rate: 60%

General Description

We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during development in C. elegans. RNAs were prepared from different time points during development of C. elegans hermaphrodites and males, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.

Protocols

  1. Growth and isolation: worm growth, worm staging and isolation, small RNA isolation
  2. Sample preparation: cDNA library prep and Illumina sequencing
  3. Other Protocols: SOAP alignment
  1. External Links: GSM336056, SRR014968


Release Date: 2009-11-18